Glioma cell proliferation is inhibited by miR-342-3p, miR-377 / E2F1 signaling pathway

 Ying Huang, Chunling Chi

Abstract:

In recent years, microRNAs (miRNAs) have been reported to be critical regulators to influence tumor genesis or its further progression by directly targeting downstream tumor related genes in glioma. However, there are still many underlying mechanisms related to miRNAs signaling pathway that remain to be uncovered. In the present study, we found that miR-342-3p and miR-377 inhibited the glioma cell line proliferation and arrested the cell cycle at G1 phase. Inhibition of miR-342-3p and miR-377 function promoted the cell proliferation. miR-342-3p and miR-377 target the E2F1 3’UTR to repress its expression on both mRNA and protein level. Downregulation of E2F1 inhibited the cell proliferation and arrested the cell cycle. Overexpression of E2F1 blocked the proliferation repression caused by miR-342-3p or miR-377 in glioma cells. This study showed the function of miR-342-3p, miR-377/E2F1 axis in regulating glioma cells proliferation and provided the potential therapeutic target.