Identification of potential crucial genes associated with vasculogenic mimicry in human osteosarcoma based on gene expression profile

Nan Yao, Ke Ren, Xiao-Jie Gu, Su-Jia Wu, Xin Shi, Qing Chang, Yong-Gang Li, Zeng-Xin Gao, Qiao-Mei Jin, Jian Zhang,  Chen Wang, Jing Zhou

Abstract:

We previously reported the presence of vasculogenic mimicry (VM) in human osteosarcoma. However, the mechanistic basis of osteosarcoma VM remains unclear. Three hundred eighty-one upregulated differentially expressed genes (DEGs) and 526 downregulated DEGs between human osteosarcoma cell line 143B and HOS cell exposed to Matrigel were screened out by microarray. GO categories such as “cell adhesion”, “angiogenesis” were enriched in 143B group. PATHWAY analysis showed enriched TGF-beta, Wnt and VEGF signaling pathway in 143B group. The hub gene ITGA2 in signal-network of DEGs exhibited pro-VM and pro-metastasis effect. Our study provides fundamental data for further studies regarding molecules involved in osteosarcoma VM.