microRNA-10a-5p overexpression suppresses malignancy of colon cancer by regulating human liver cancer fibroblasts

Xuan Zheng, Jing-Wu Li, Yan-Kun Liu, Yi-Fu Ma, Jian-Hui Gan, Su-Gui Han, Jian Wang, Zhao-Yuan Wan, Jun Zhang, Yan Liu,  Yu-Feng Li,  Guang-Ling Zhang

Abstract:

The crosstalk between tumor and stroma plays a critical role in cancer metastasis. However, the function of miR-10a-5p on liver fibroblasts in the metastatic microenvironment of colon cancer (CC) and the effect of activated fibroblasts on CC cells are still unclear. In our study, miR-10a-5p overexpression inhibited the proliferation, migration, and IL-6/IL-8 level of LX-2 cells and human liver cancer fibroblasts (HLCFs). Moreover, miR-10a-5p had lower expression in HLCFs than in human liver normal fibroblasts (HLNFs). The conditioned medium (CM) from LX-2 cells with miR-10a-5p overexpression or HLNFs could inhibit the invasion, migration, and stemness of CC SW480 cells, whereas HLCFs CM could promote these malignant phenotypes of SW480 cells. The present study illustrates the effect of miR-10a-5p on the liver fibroblasts and the altered liver fibroblasts in the microenvironment on CC cells induced by miR-10a-5p, which may aid the understanding of the mechanisms underlying CC liver metastasis.