Garlic-derived organosulfides induce cytotoxicity, apoptosis, cell cycle arrest and oxidative stress in human colon carcinoma cell lines
Abstract:
Organosulfur compounds (OSC) from garlic, especially allicin (ALI), diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS) are recognized as a group of potential chemopreventive agents. In this study, we examined the effects of OSC on human Caco-2 and HT-29 colon carcinoma cell lines. Apoptosis induction (Annexin-V-FITC/PI, fluorescein diacetate/PI, sub-G1 fraction), modulation of DNA cell cycle (G2/M arrest, phospho-H3 mitotic marker), transmembrane mitochondrial potential (JC-1) and intracellular GSH amount (monochlorobimane assay) were measured by flow cytometry and fluorimetry. Our results showed that order of OSC-induced cell death in Caco-2 and HT-29 cells increased in the range as follows: ALI