MicroRNA-520f suppresses growth of gastric carcinoma cells by target ATPase family AAA domain-containing protein 2 (ATAD2)
Abstract:
MicroRNAs (miRNAs) are small, non-coding RNAs that can serve as tumor suppressor genes or oncogenes in tumorigenesis. More and more evidence demonstrate that abnormal expression of miRNAs lead to the gastric carcinoma occurrence. In the present study, we revealed that the expression levels of miR-520f were significantly down-regulated in gastric carcinoma cells and clinical gastric carcinoma samples. Next, we demonstrated that introduction of miR-520f inhibited the growth of gastric carcinoma cells in vitro. However, down-regulate the expression levels of miR-520f by anti-miR-520f lead to an enhanced cell proliferation, implying that miR-520f maybe serve as a novel tumor suppressor. Moreover, we found that ATPase family AAA domain-containing protein 2 (ATAD2) was one target genes of miR-520f downstream regulator, which caused the decreased expression of ATAD2. Meanwhile, the overexpression of ATAD2 reversed the inhibited proliferation ability caused by miR-520f. Therefore, our find that miR-520f involves in gastric carcinoma proliferation, pointing a therapeutic probability of miR-520f in the therapy of gastric carcinoma.