Potential key molecular correlations in laryngeal squamous cell carcinoma revealed by integrated analysis of mRNA, miRNA and lncRNA microarray profiles
Abstract:
To uncover potential key genes, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) associated with laryngeal squamous cell carcinoma (LSCC), microarray data of mRNA, miRNAs and lncRNAs produced from matched sample pairs of LSCC and adjacent normal samples were used to this analysis. Differentially expressed genes (DEGs), miRNAs (DE-miRNAs) and lncRNAs (DE-lncRNAs) were identified, and functions and correlations of them were analyzed. In total, 826 DEGs, 44 DE-miRNAs and 347 DE-lncRNAs were identified. The up-regulated DEGs were mainly related to cell cycle, and the down-regulated DEGs were correlated with regulation of biological quality and extracellular region. Furthermore, ATP1A2 was regulated by the lncRNA FLJ42875; AQP1 and TGFBR2 were targeted by LOC100505976; genes like BUB1B and CENPE were modulated by XLOC_l2_010636. Besides, genes like FGF2 and PIK3R1, and lncRNAs like LOC100505976 and XLOC_l2_010636 were modulated by hsa-miR-424-5p. The expression levels of hsa-miR-424-5p, LOC100505976 and XLOC_l2_010636 as well as several DEGs were confirmed by quantitative real time polymerase chain reaction. These regulatory relationships of DEGs, DE-miRNAs and DE-lncRNAs might play pivotal roles in the tumorigenesis of LSCC.