Urinary fluorescence analysis in diagnosis of bladder cancer

 Boris Kollarik, Milan Zvarik, Peter Bujdak, Peter Weibl, Lubos Rybar, Libusa Sikurova, Luba Hunakova

Abstract:

Early diagnosis of bladder cancer is crucial for improvement of cancer specific survival and recurrence rate. We analyzed the possible role of fluorescence urine analysis in bladder cancer diagnosis. The cohort consisted of 20 healthy patients, 40 patients with hematuria and 75 patients with hematuria and histologically proven bladder tumor. Synchronous fluorescence spectra with a 70 nm wavelength difference were recorded for (1:1 – 1:128) urine dilutions. Concentration matrices of synchronous spectra (CMSS) were used to classify samples into tested groups. CMSS analysis allowed us to distinguish patients with tumor from patients with hematuria with a sensitivity 55% and specificity 74.7%. This is comparable to the sensitivity and specificity of other noninvasive tests like BTA stat and NMP-22 (Bladder check®). Lower fluorescence intensity of Imax 280 nm and ratio of 280 nm to 450 nm was found to be associated with the presence of tumor. We have found an association of decreased fluorescence with the stage of the disease. Our data suggest that CMSS urine analysis has a potential role in the non-invasive diagnostic tests for bladder cancer, but it cannot replace the current diagnostic algorithm yet.