miR-497 inhibits tumor growth and migration of osteosarcoma by targeting plexinA4 and CDK6

Zhi-Sen Tian, Meng-Jie Yan, Shuo Li, Dan Cong, Yuan-Yi Wang,  Qing-San Zhu

Abstract:

MicroRNAs are small non-coding RNAs that regulate gene expression at the post-transcriptional level which have been reported to be involved in the pathogenesis of various cancers. In the present study, we found that miR-497 was downregulated in osteosarcoma tissues. Gain and loss of function studies were carried out to investigate the effect of miR-497 on the growth of osteosarcoma cells. The results indicated that miR-497 inhibited the growth of osteosarcoma cells. Furthermore, bioinformatics analysis predicted plexinA4 and CDK6 as targets of miR-497, which was afterward confirmed by luciferase activity assay and rescue experiments. These findings suggested that miR-497, plexinA4, and CDK6 may serve as novel potential makers for osteosarcoma diagnostics and therapy.